In this room, there is no incubation tank like there is in the first and and second vaults. Instead, there is an upper walkway housing two portals. When Samus first enters this room on her way to the Stronghold Void boss, she will have to destroy a Crash Pillar, Electro Voldrums, Guardians, and Psycho Bits in the room. Samus will later obtain the Shock Coil in this room.
- Shock Coil
- Samus must drop down through the side passage when entering from Incubation Vault 01 to fall next to the Shock Coil.
- ""TELEPORTATION DEVICE ONLINE. STEP INTO THE PORTAL TO WARP TO ANOTHER LOCATION."
- Blue Force Field
- "ANALYSIS INDICATES THE FORCE FIELD IS IMPERVIOUS TO MOST WEAPONS. A CONCENTRATED BLAST OF NEUTRINOS MAY DAMAGE ITS GUARD NODE."