This is the only access to Life Grove, where one of the Chozo Artifacts is hidden. The room is an oval shaped tunnel, with two smaller tunnels leading out; the left from Great Tree Hall and the right to the Life Grove. The entrance to the tunnel is blocked by Bendezium. The oval tunnel loops round a smaller oval in the centre. In here, Samus must use her Morph Ball and Boost Ball ability to boost up the sides of the tunnel. This room also contains a Missile Expansion.
- Missile Expansion
- Samus can obtain this Expansion by boosting to the top of the central oval. In the middle, she must lay a bomb to reveal a secret tunnel leading to the bottom of Life Grove Tunnel's main Half-pipe. If Samus rolls down the hole inside the central oval, she will be rewarded with the Expansion.
- "Analysis of wall shows rampant weakness due to severe water saturation. Large traces of Bendezium detected."