Main Ventilation Shaft Section E is part of a network of ventilation shafts in the Frigate Orpheon in Metroid Prime that Samus travels through after the Parasite Queen battle in order to escape the frigate. This section opens into a humid section with a large metal cylinder that repeatedly retracts and returns. It does not harm Samus if she is in front when it pushes forward; it will simply push her back. Waiting for it to retract far enough reveals the door to the next part of the shaft.
- Main Ventilation Shaft Section E, along with Main Ventilation Shaft Sections D and F, may or may not have survived the crash into Tallon IV, but it is impossible know for sure due to a huge rock blocking the door in C, leading to D, leading to E.