The Multiviola Nest is constructed primarily from green blocks, with a few silver pipes. It is a horizontal corridor with a narrow middle section. There is a passage beneath the corridor that avoids most of the enemies, although it is flooded with lava in Metroid and is therefore not very useful to Samus Aran.
In Metroid, the appropriately-named Multiviola Nest is infested with Multiviolas, constantly bouncing off of surfaces for the entire length of the corridor. The close quarters often make it difficult to dodge the Multiviolas or use the Screw Attack. However, since Multiviolas often drop large amounts of Energy or Missiles when killed, this room is useful for replenishing Samus's stock.
In Zero Mission, the Multiviola Nest ironically no longer has Multiviolas, and it is much shorter in length due to the addition of a new room to the left. Three Air Holes are home to Zebbos, while three Holtz lurk above. The right end of the room is blocked off by a Pit Block, so Samus can only fully traverse the room from left to right; if she enters through the Blue Door on the right, she is stopped by a dead end. Additionally, falling into one of the pits in the room directly above will drop her into the right side of the Multiviola Nest.
In both games, a Blue Door on the right leads to the rightmost vertical shaft in Ridley's Lair. In Metroid, the Blue Door on the left connects to a small fuchsia shaft that cannot be ascended. In Zero Mission, the Blue Door on the left connects to a room with a slope and many enemies caged by Boost Blocks. In Zero Mission, a room with destructible pipe bridges has a one-way pitfall through Pit Blocks into the Multiviola Nest.
- 25 Multiviolas
- Energy Tank
- In Zero Mission, Samus can bomb the floor and enter the room's Secret Passage. Directly below the middle Air Hole is an Energy Tank sitting on a pedestal.
"This is a Multiviola Nest!! Use it to collect Energy."
- Although it is referred to as a nest, the Multiviola Nest does not feature any larval Violas in either game.