It first appeared as Crateria - The Space Pirates Appear (クレテリア 宇宙海賊出現) in Super Metroid, which is heard when Samus is ambushed by Zebesians in Tourian's ruined Zebesian Command Center. After this sequence, it is also heard in old Brinstar and nearly all of the left part of Crateria. It will also play in the far-right caverns of Crateria if Samus enters them from Maridia, despite the complete lack of Pirates there. This theme is included on the Super Metroid: Sound in Action CD as an arranged version, which includes Planet Zebes - Arrival on Crateria, another Super Metroid track.
Two different variations are heard during Metroid: Zero Mission, when Zero Suit Samus is forced to escape Zebes through Chozodia (including a section of the Wrecked Ship) and the Space Pirate Mother Ship. The former version plays during the stealth sequences, while the latter version plays when Zebesians spot her and is included as number 20 in the Sound Test. Both versions are permanently replaced by Brinstar (Metroid) once the Ruins Test has been completed.
This theme is heard in Pirate Command in Metroid Prime 3: Corruption, and is one of the songs available for purchase in the Soundtrack Gallery. Unlike most other versions of the theme, this one has a deeper, more electronic-sounding instrumentation and tone. Pirate Homeworld also does not use the recognisable melody from the Space Pirates Appear until about 26 seconds in. On the UK Corruption website, it is known as Track_10 in the music player.
The original theme has been performed in orchestra twice; first by the Tokyo Symphony Orchestra in 1994 as part of a medley (which arranges The Space Pirates Appear in a more lighthearted manner), and then in 2013 by the WDR Radio Orchestra as part of Into Red, Into Dark.
- In Zero Mission, if Samus manages to sneak through Chozodia without ever getting detected by Space Pirates, neither version of this theme will ever be heard.