|This article is written from the Real Life point of view|
- This article is about the theme from Metroid Prime. For the theme from Samus Returns, see Theme of Samus.
It is heard at the beginning of the game, as Samus Aran is landing her Gunship on the Frigate Orpheon. The theme begins slow, before breaking into a partial remix of the Theme of Samus when Samus's Gunship appears. The theme ends a few seconds after Samus steps out onto the Exterior Docking Hangar, and leads into her Appearance Fanfare. After this, she becomes playable for the first time in 3-D. On the soundtracks, the appearance fanfare is merged into the Prologue track, while also appearing as a separate track.
The Prologue remix of Theme of Samus also plays briefly in the Special Ending of Metroid Prime 2: Echoes as Samus’s gunship is leaving Aether. This version starts with the Theme of Samus, and then transitions to a low choir singing The Luminoth.
- The beta version of Prologue from Metroid Prime sounded softer and more somber in tone: 
- This theme was originally accompanied by a female narrator recounting the Zero Mission "10 years ago" and Samus's detection of the Orpheon distress signal. This voiceover is unused, but still accessible in the internal files. The original PAL and New Play Control! versions of Prime accompany the Prologue with a male narrator (voice actor unknown) speaking about the legend of Samus Aran.