|This article is written from the Real Life point of view|
In Return of Samus, Title begins with nearly a whole minute of a repeating high-pitched dissonant chord. As it continues, static-like sounds and heavy beats are added to the background. Eventually, a melody briefly plays, which starts off similar to Silence but then diverges into a new and unique tune.
Finally, a soft and lulling theme begins to play. This serene melody is comparable to the original Title (Metroid), which similarly starts off tense and then transitions to a more relaxing lullaby-like tune. As it continues, the same heavy beats from before start playing once again.
It then builds up to a big climax: a triumphant fanfare punctuated by the heavy beats. After a few repetitions, the fanfare fades away to silence, while the echoing beats continue.
In Samus Returns, much of the original theme is intact. However, it now opens with a remix of the six-note leitmotif from Title (Metroid), and then goes straight to the serene melody, which has been abridged. The original opening is still present in this remix, now moved to the end of the song (much like Metroid: Zero Mission); interestingly, the repeating dissonant chords retain their original chiptune sound.
In Return of Samus, a more subdued version of Title's triumphant fanfare is heard when first starting the game and Samus Aran appears next to her Gunship in Phase 1. It plays again whenever a saved game is loaded and Samus appears at a Save Point. Essentially, this serves as this game's version of Samus Aran's Appearance Fanfare, which does not appear in this game at all. It can be heard here: 
In Samus Returns, a portion of Title's melody is a recurring motif in the game. It is heard in the exotic jungle music that plays in the lowest section of Area 5, known internally as Area Indefinite:  It is heard again in the Area 8 theme, where it sounds heavily distorted and sinister: 
In the Samus Returns remix of Staff Credit, Title can be heard twice. The aforementioned recurring motif is played halfway through the track, just after the original Return of Samus Staff Credit theme ends. The serene melody is then heard later, nestled between Theme of Samus Aran, Space Warrior and Surface of SR388. It can be heard here: 
- The original Title theme of Return of Samus is one of the only title themes that does not incorporate the series's main theme: the six-note leitmotif heard at the start of Title (Metroid). This was rectified in Samus Returns.
- The Samus Returns version of the Title theme appears in the trailer for the Nintendo Switch port of Rocket League, as the car based on Samus's color scheme is introduced.